硕士生导师

姓名：厉萍

职称：副研究员

邮箱:liping2018@email.sdu.edu.cn

专业领域：海洋科学、资源与环境

研究方向：环境生物学、分子生物学、蛋白组学、低温冷冻生物学

讲授课程：分子生物学、基因工程、分子育种学、现代生物学实验技术指导

主要致力于河流及近海水环境污染物分布特征、典型污染物对水生生物潜在的毒性效应及分子机制、以及生态风险评估等研究。曾先后主持国家自然基金青年项目、捷克自然基金项目、科技部重点研发计划子任务、山东省自然基金面上项目、中央院所基本科研业务费等多项课题。先后发表学术论文70余篇，其中第一作者（或通讯作者）33篇，参编中英文专著4部，获得授权发明专利3项。此外，还担任BioMed Research International专刊客座编辑，以及PloS ONE; BioMed Research International; Reproduction, Fertility and Development; Fish Physiology and Biochemistry等SCI期刊的独立审稿人。

邮箱: liping2018@email.sdu.edu.cn

教育工作经历

• 2004年7月毕业于山东师范大学生命科学学院，获学士学位。

• 2007年7月获华中农业大学水产学院湿地资源与环境专业，获硕士学位。

• 2007年10月至2011年10月赴捷克南波希米亚大学水产养殖与水资源保护学院从事鱼类精液超低温冷冻保存技术及精子冷冻损伤机制研究，并获得博士学位；曾赴日本筑波大学下田海洋研究中心实验室访问交流（2009年1-4月）；在捷克获得由国家留学基金委颁发的2010年度国家优秀自费留学生奖学金。

• 2011年11月至2012年11月赴新加坡国立大学生物系从事博士后研究工作，主要运用代谢组学和iTRAQ标记的蛋白质组学研究水体污染物对鱼类影响的分子机制。

• 2007年7月至2018年4月任职于中国水产科学研究院长江水产研究所，2014年晋升副研究员，2015年获第三批“中国水产科学研究院百名科技英才培育计划”人选。

• 2018年5月至今 山东大学（威海），澳门永利总站注册送38。

主持科研项目

• 国家重点研发计划“蓝色粮仓科技创新”专项, 2018YFD0900905，“我国重要渔业水域食物网结构特征与生物资源补充机制”项目-“关键资源种群衰退与修复机理”，2018/12-2022/12，任务负责人

• 山东省自然科学基金面上项目，ZR2019MC011，喹诺酮类抗生素对鲤鱼生殖内分泌干扰作用研究，2019/01-2021/12

• 浙江省淡水水产研究所，农业部淡水渔业健康养殖重点实验室开放课题，ZJK201913，喹诺酮类抗生素对鲤鱼肝和肾胁迫效应研究，2019/01-2020/12

• Czech Science Foundation, GACR 16-02407Y, Tf in the seminal plasma of carp and some sturgeons can help us to identify the role of proteins in the freezing of sperm.2016/01-2018/12

• 国家自然基金青年项目，31402301，鲟鱼精子质量蛋白质标志物的筛选与功能研究，2015/01-2017/12

• 中央级公益性科研院所基本科研业务费优青项目，2015B02YQ01，水产生物生殖调控相关机制及技术研究-达氏鲟精原干细胞体外增殖与冷冻保存，2015/01- 2017/12

• 中国水产科学研究院“院5511人才工程百名科技英才培育计划”人选，2015年，渔业资源保护与利用学科

• Grant agency of University of South Bohemia, 003/2010/Z, Effect of cryopreservation on phosphorylation state of sperm proteins of common carp,Cyprinus carpioL, 2010/01-2010/12

• Grant agency of University of South Bohemia, 072/2008/P, Ice-age endurance: difference expressions of sperm proteins before and after cryopreservation in teleost fishes, 2009/01-2009/12

代表性论文:

1. Li, P., Li, Z.H.*,2021. Neurotoxicity and physiological stress in brain of zebrafish chronically exposed to tributyltin. Journal of Toxicology and Environmental Health, Part A Current Issues, 84, 20-30.

2. Li Z.H.*,Li P.*,Wu Y.H., 2021. Effects of temperature fluctuation on endocrine disturbance of grass carpCtenopharyngodon idellaunder mercury chloride stress. Chemosphere. 263: 128137.

3. Li, Z.H.*,Li, P*.2021. Effects of the tributyltin on the blood parameters, immune responses and thyroid hormone system in zebrafish.Environmental Pollution, 268, 115707.

4. Li Z.H.*,Li P.*,Wu Y.H., 2021. Regulation of glutathione-dependent antioxidant defense system of grass carpCtenopharyngodon idellaunder the combined stress of mercury and temperature. Environmental Science and Pollution Research, 28, 1689-1696.

5. Li, P., Li, Z.H.*,2020. Tributyltin induces the tissue-specific stresses in zebrafish, a study in various tissues of muscle, gill and intestine. Bulletin of Environmental Contamination and Toxicology, 105, 847–852.

6. Li P., Li Z.H.*, Wu Y.H.,2020. Interactive effects of temperature and mercury exposure on the stress-related responses in the freshwater fishCtenopharyngodon idella. Aquaculture Research,00: 1-8.DOI: 10.1111/are.15058

7. Li P.,Li Z.H.*, 2020. Toxicity evaluation of triphenyltin in zebrafish larvae by embryonic malformation, retinal development, and GH/IGF axis. Fish Physiology and Biochemistry.46, 2101-2107

8. Li P.,Li Z.H.*, 2020. Environmental co-exposure to TBT and Cd caused neurotoxicity and thyroid endocrine disruption in zebrafish, a three-generation study in a simulated environment. Environmental Pollution. 259: 113868.

9. Li P.,Li Z.H.*, Zhong L., 2020.Parental exposure to triphenyltin inhibits growth and disrupts thyroid function in zebrafish larvae.Chemosphere. 240: 124936.

10. Li P.,Li Z.H.*, Zhong L., 2019.Effects of low concentrations of triphenyltin on neurobehavior and the thyroid endocrine system in zebrafish.Ecotoxicology and Environmental Safety. 186: 109776.

11. Li P.,Li Z.H.*,Zhong L., 2019.Triphenyltin exposure alters the antioxidant system, energy metabolism and the expression of genes related to physiological stress in zebrafish (Danio rerio).Comparative Biochemistry Physiology Part C Toxicology and Pharmacology. 225: 108581.

12. Li P.,*, Du H, Qiao X.M., Liu Z.G., Zhou Q., Wei Q.W. *, 2019. Protein profile of Dabry's Sturgeon (Acipenser dabryanus) spermatozoa and relationship to sperm quality. Animal Reproduction Science. 201: 1-11.

13. Xie X.#,Li P.#, Pšenička M., Ye H., Steinbach C., Li C., Wei Q.*, 2019.Optimization of in vitro culture conditions of sturgeon germ cells for purpose of surrogate production.Animals (Basel). 9(3). pii: E106.

14. Li P*., Guo W., Qiao X.M., Liu Z.G., Shen L., Wei Q.W.*, 2019.Morphology and ultrastructure of Dabry’s sturgeon (Acipenser dabryanus) spermatozoa usingscanning and transmission electron microscopy. Journal of Applied Ichthyology. 35: 192-201.

15. Xi M.D.#,Li P.#, Du H., Qiao X.M., Liu Z.G., Wei Q.W.*, 2018. Disaccharide combinations and the expression of enolase3 (ENO3) and plasma membrane Ca²⁺ATPase isoform (PMCA2) in sturgeon sperm cryopreservation. Reproduction in Domestic Animals. 53: 472-483.

16. Li P., Xi M.D., Du H., Qiao X.M., Liu Z.G., Wei Q.W.* 2018. Antioxidant supplementation, effect on post-thaw spermatozoon function in three sturgeon species. Reproduction in Domestic Animals. 53: 287-295.

17. Li P., Guo W., Yue H.M., Li C.J., Du H., Qiao X.M., Liu Z.G., Zhou Q., Wei Q.W. *. 2017. Variability in the protein profiles in spermatozoa of two sturgeon species.PLoS One.12(10): e0186003.

18. Li Z.H.*,Li P.*, Shi Z.C. 2016. Chronic effects of tributyltin on multiple biomarkers responses in juvenile common carp,Cyprinus carpio. Environmental Toxicology. 31(8), 937-944.

19. Li Z.H.*,Li P.*, Shi Z.C. 2016.Physiological and molecular responses in brain of juvenile common carp (Cyprinus carpio) following exposure to tributyltin. Environmental Toxicology. 31(3), 278–284.

20. Li P.,Li Z.H*. 2015. Physiological responses in Chinese rare minnow larvae following exposure to low-dose tributyltin. Bulletin of Environmental Contamination and Toxicology, 95: 588–592.

21. Li Z.H.*,Li P.*,Shi Z.C. 2015.Responses of the hepatic glutathione antioxidant defense system and related gene expression in juvenile common carp after chronic treatment with tributyltin. Ecotoxicology, 24, 700-705.

22. Li, P.*, Hulak, M., Li, Z.H., Sulc, M., Psenicka, M., Rodina, M., Gela, D., Linhart., O., 2013. Cryopreservation of common carp (Cyprinus carpioL.) sperm induces protein phosphorylation in tyrosine and threonine residues.Theriogenology, 80(2): 84-89.

23. Li, P.*, Li, Z.H.*, Hulak, M., Rodina, M., Linhart, O., 2012.Regulation of spermatozoa motility in response to cations in Russian sturgeon (Acipenser gueldenstaedtii). Theriogenology 78(1): 102-109.

24. Li, Z.H.^*,Li, P.^*, Sulc, M., Hulak, M., Randak, T., 2012.Hepatic proteome sensitivity in rainbow trout after chronically exposed to a human pharmaceutical Verapamil. Molecular and Cellular Proteomics, 11(1): M111.008409.

25. Li, P.*, Rodina, M., Hulak, M., Li, Z.H., Linhart, O., 2011.Spermatozoa concentration, seminal plasma composition and their physiological relationship in the endangered stellate sturgeon (Acipenser stellatus) and Russian sturgeon (Acipenser gueldenstaedtii). Reproduction in Domestic Animals 46(2): 247-252.

26. Li, P.*, Rodina, M., Hulak, M., Gela, D., Psenicka, M., Li, Z.H., Linhart, O., 2011.Physico-chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula). Journal of Applied Ichthyology 27(2): 673-677.

27. Li, P.^*, Li, Z.H., Dzyuba, B., Hulak, M., Rodina, M., Linhart, O., 2010. Evaluating the impacts of osmotic and oxidative stress on common carp (Cyprinus carpio, L.) sperm caused by cryopreservation techniques.Biology of Reproduction 83(5): 852-858.

28. Li, P.*, Dzyuba, B., Hulak, M., Rodina, M., Boryshpolets, S., Li, Z.H., Linhart, O., 2010. Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity. Theriogenology, 74(8): 1356-1361.

29. Li, P.*, Hulak, M., Koubek, P., Sulc, M., Dzyuba, B., Boryshpolets, S., Rodina, M., Gela, D., Manaskova-Postlerova, P., Peknicova, J., Linhart., O., 2010. Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp,Cyprinus carpioL. Theriogenology 74(3): 413-423.

30. Li, P.*, Hulak, M., Rodina, M., Sulc, M., Li, Z.H., Linhart, O., 2010.Comparative protein profiles: potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces).Comparative Biochemistry and Physiology: Part D: Genomics and Proteomics5(4): 302-307.

31. Li, P.*, Rodina, M., Hulak, M., Gela, D., Li, Z.H., Linhart, O., 2010.Physico-biochemical parameters and protein profiles of sperm from belugaHuso huso. Journal of Applied Ichthyology 26(5): 753-755.

32. Li, P., Hulak, M., Linhart, O.*, 2009.Sperm proteins in teleostean and chondrostean (sturgeon) fishes. Fish Physiology and Biochemistry 35(4):567-581.

33. Li, P., Wei, Q.*, Liu, L., 2008. DNA integrity of Polyodon spathula cryopreserved sperm. Journal of Applied Ichthyology 24(2): 121-125.